Abstract The effect of commercial Atrazine (ATR) on Rattus Novergicus organs was determined for a concentration of cATRof 1, 3, 13, 30, and 50 ppb. ATR was dissolved in drinking water. The rats were allowed to drink from it ad libitum for an uninterrupted period of 28 days as established by the Office of Environmental Protection protocol under the number EPA OPPTS 870.3050. In the 28 days of the test, data on the behavior of the medicated animals was recorded before the extraction of sample tissues (heart, liver, spleen, brain, and testicles) for histological analysis. A direct correlation of cATRand organ damage was found. The study showed that even for the smallest doses (1ppb), commercial ATR produced several lesions in the studied animals. The rats showed hepatic periacinar necrosis with acute coagulation, hepatocyte lipidosis, severe portal lymphocytic inflammation, coronary periarteritis, and lymphocytic meningoencephalitis for high concentrations. In the male reproductive system, testicular degeneration with mild acute tubular necrosis was observed.

ABSTRACT Overweight in school-age children represents a growing public health problem in Panama. The objective is to develop and validate an intervention program in food and nutrition education (FNE) against overweight and obesity in school-age children in Panama from the perspective of an intersectoral public policy in health and education. The intervention is based on behavior changes under the socio-ecological model that favors the adoption of healthier dietary practices and lifestyles. This is a study in 362 school-age children of quasi-experimental, pre-test and post-test, prospective and non-controlled design, in public education institutions in three districts of Panama West Province: La Chorrera, Capira and Chame. Five schools were selected: the Victoria D’Spinay and La Herradura schools in the District of La Chorrera, the Hernando Bárcenas school in the District of Capira and the El Espavé and Berta Elida Fernández schools in the Chame District. The protocol was registered in the international test platform N° ISRCTN28920505. Sociodemographic, anthropometric, nutritional and food data will be obtained; of food security; and of knowledge, attitude, perception and behavior on food and healthy lifestyles from March to December 2019 during the implementation of the intersectoral program called “Healthy Schools” of the Ministry of Health and the Ministry of Education of Panama. The data will be described and compared using parametric and non-parametric statistics. The study protocol was reviewed and approved by the Bioethics Committee of the University of Panama (Ref. CBUP/Nº075/2019), registered in the General Directorate of Health (DIGESA) of the Ministry of Health (Nº 881). Signed informed consent will be requested from the guardians and assent for each participating child. The results of the study will be widely disseminated in peer-reviewed journals and in health and nutrition congresses.

RESUMEN El sobrepeso en escolares representa un creciente problema de salud pública en Panamá. El objetivo es desarrollar y validar un programa de intervención en educación alimentaria y nutricional (EAN) contra el sobrepeso y la obesidad en escolares de Panamá bajo la perspectiva de una política pública intersectorial de salud y educación. La intervención se basa en el cambio de conducta bajo el modelo socio-ecológico que favorezca la adopción de comportamientos alimentarios y de estilos de vida más saludables. Se trata de un estudio en 362 escolares de diseño cuasi experimental, pre-post intervención, prospectivo y sin grupo control, de tipo observacional explicativo en instituciones de educación básica general del sector público en tres Distritos de Panamá Oeste: La Chorrera, Capira y Chame. Se seleccionaron 5 escuelas: la escuela Victoria D’Spinay y La Herradura en el Distrito de La Chorrera, la escuela Hernando Bárcenas en el Distrito de Capira y las escuelas El Espavé y Berta Elida Fernández en el Distrito de Chame. El protocolo fue registrado en la plataforma internacional de ensayos Nº ISRCTN28920505. Se obtendrán datos sociodemográficos, antropométricos, nutricionales y alimentarios; de seguridad alimentaria; y de conocimiento, actitud, percepción y comportamiento sobre alimentación y estilos de vida saludables de marzo a diciembre de 2019 durante la implementación del programa intersectorial denominado “Escuelas Saludables” del Ministerio de Salud y del Ministerio de Educación de Panamá. Se describirán y compararán los datos utilizando estadística paramétrica y no paramétrica. El protocolo de estudio fue revisado y aprobado por el Comité de Bioética de la Universidad de Panamá (Ref. CBUP/Nº 075/2019), registrado en la Dirección General de Salud (DIGESA) del MINSA (Nº 881). Se solicitará consentimiento informado firmado por parte de los acudientes y asentimiento por cada niño participante. Los resultados del estudio serán difundidos ampliamente en revistas indexadas de revisión de pares y en congresos de salud y nutrición.